Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT1A/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist 5-HT. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of 5-HT (Mean ± SD, n = 2). The EC₅₀ of 5-HT on this cell was 17.7 nM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2 10 μg of membranes prepared from CHO-K1 cells stably expressing 5-HT1A receptors were incubated with indicated concentrations of [³H]5-HT in the absence (total binding) or presence of 1000-fold access unlabeled Serotonin (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

Figure 3 10 μg of membranes prepared from CHO-K1 cells stably expressing 5-HT1A receptors were incubated with indicated concentrations of 5-HT in the presence of 2 nM [³H]5-HT. Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.

CHO-K1/5-HT1A/Gα15 Stable Cell Line

The 5-Hydroxytryptamine receptor 1A (5-HT1A) is Gi-coupled GPCRs expressed in the cerebral cortex, hippocampus, septum, amygdala, and raphe nucelus, with lesser amounts in the basal ganglia and thalamus. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. 5-HT1A ligands may prove to be therapeutic in the treatment of various disorders such as depression, anxiety, and schizophrenia.

產品編號	M00330
規格	2 vials 大單詢價
數量
價格	詢價


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Synonyms

Human Recombinant 5-HT1A Serotonin Receptor Stable Cell Line

產品簡介

Storage

Liquid nitrogen immediately upon delivery

培養條件

Culture Medium	Ham’s F-12, 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies)
Freeze Medium-DATA	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)

圖例

Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT1A/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist 5-HT. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of 5-HT (Mean ± SD, n = 2). The EC₅₀ of 5-HT on this cell was 17.7 nM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2 10 μg of membranes prepared from CHO-K1 cells stably expressing 5-HT1A receptors were incubated with indicated concentrations of [³H]5-HT in the absence (total binding) or presence of 1000-fold access unlabeled Serotonin (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

Figure 3 10 μg of membranes prepared from CHO-K1 cells stably expressing 5-HT1A receptors were incubated with indicated concentrations of 5-HT in the presence of 2 nM [³H]5-HT. Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.

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CHO-K1/5-HT1A/Gα15 Stable Cell Line

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