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目錄產品 » 穩定細胞系 » Human Recombinant P2Y2 Purinergic Receptor Stable Cell Line

Figure 1: ATP-induced concentration-dependent stimulation of intracellular calcium mobilization in 1321N1/P2Y2 cells. The cells were loaded with Calcium-4 prior to stimulation with a P2Y2 receptor agonist, ATP. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of ATP (Mean ± SEM, n = 3). The EC₅₀ of ATP on P2Y2 in 1321N1 cells was 32.38 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

1321N1/P2Y2 Stable Cell Line

Recombinant 1321N1 cells stably overexpress human purinergic receptor P2Y (P2Y2) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.

產品編號	M00318
規格	2 vials 大單詢價
數量
價格	詢價


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產品簡介

Product Description	Recombinant 1321N1 cells stably overexpress human purinergic receptor P2Y (P2Y2) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養條件

Culture Medium	DMEM, 10% FBS, 250 μg/ml G418 (Cat. No. 10131-035, Gibco)
Complete Growth Medium	DMEM, 10% FBS
Freeze Medium-DATA	45% DMEM (Cat. No. 10569, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)