Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT2A cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist 5-HT. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of 5-HT (Mean ± SEM, n = 3). The EC₅₀ of 5-HT on this cell was 6.15 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/5-HT2A Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human 5-hydroxytryptamine receptor 2A (5-HT2A) on the surface and contain high levels of G protein Gαq and Gαi to couple with the receptor in downstream signaling pathways.

產(chǎn)品編號(hào)	M00251
規(guī)格	2 vials 大單詢(xún)價(jià)
數(shù)量
價(jià)格	詢(xún)價(jià)


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產(chǎn)品簡(jiǎn)介

Product Description	Recombinant CHO-K1 cells stably overexpress human 5-hydroxytryptamine receptor 2A (5-HT2A) on the surface and contain high levels of G protein Gαq and Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養(yǎng)條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

圖例

Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT2A cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist 5-HT. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of 5-HT (Mean ± SEM, n = 3). The EC₅₀ of 5-HT on this cell was 6.15 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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CHO-K1/5-HT2A Stable Cell Line

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