Figure 1: Neurotensin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NTS1 cells. The cells were loaded with Calcium-4 prior to being stimulated with the agonist, neurotensin. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of neurotensin (Mean ± SEM, n = 2). The EC₅₀ of neurotensin on this cell was 7.4 nM.
Note:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom

CHO-K1/NTS1 Stable Cell Line

Neurotensin receptor 1 (NTS1) is a member of G-protein coupled receptor family A and is a receptor for Neurotensin (NT). Neurotensin (NT) exerts its intracellular effect by interacting with 3 different receptors. Two of these receptors (NTR1 and NTR2) belong to the G protein-coupled receptor family, whereas the third one (NTR3) is a type I receptor with a single transmembrane domain. The NTS1 is expressed in CNS such as cerebral cortex, basal ganglia, limbic areas, vestibular system, and esophageal smooth muscle.

產(chǎn)品編號(hào)	M00194
規(guī)格	2 vials 大單詢(xún)價(jià)
數(shù)量
價(jià)格	詢(xún)價(jià)


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Synonyms

Human Recombinant NTS1 Neurotensin Receptor Stable Cell Line

產(chǎn)品概述

Applications

Functional assay for NTS1 receptor

產(chǎn)品簡(jiǎn)介

Storage	Liquid nitrogen immediately upon delivery
Species	Human

培養(yǎng)條件

Freeze Medium	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco)

圖例

Figure 1: Neurotensin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NTS1 cells. The cells were loaded with Calcium-4 prior to being stimulated with the agonist, neurotensin. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of neurotensin (Mean ± SEM, n = 2). The EC₅₀ of neurotensin on this cell was 7.4 nM.
Note:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom

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CHO-K1/NTS1 Stable Cell Line

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