| ￥1050 | |
| RC00008 | |
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| Description | PE6d is designed to optimize the efficiency of prime editing by altering certain components of the prime editor framework. It incorporates elements that minimize the chances of errors and maximize successful genomic edits across various cell types. This improvement results in enhanced editing accuracy and reduced byproducts, such as unintended indels. |
| single guide RNA (sgRNA) dependent single-stranded DNA cleavage |
| References | David R Liu, et al. “Phage-assisted evolution and protein engineering yield compact, efficient prime editors”. Cell. 2023 Aug 31;186(18):3983-4002.e26. |
| Purity | ≥90% by SDS-PAGE, 97% by SEC-HPLC |
| Residual DNase | Non-specific DNase activity |
| Endotoxin Level | Control <10EU/mg |
| Expression System | Recombinant Cas9-based fusion with three NLS expressed by E.coli |
| Species | S. pyogenes |
| Tag | C-His tag |
| Molecular Weight | 223.780 KDa |
| Storage & Stability | PE6d is supplied with storage buffer (50 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.5, at 25°C) and recommended to be stored at -80°C. |
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