| ￥1400 | |
| RC00005 | |
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| Description | PEmax is an improved version of PE2 with additional NLS and improved nCas9 domain, facilitating precise genome editing without the need for double-stranded DNA breaks. It significantly increases editing efficiency and accuracy compared to earlier systems and allows for a broader range of base conversions and edits within the genome design |
| single guide RNA (sgRNA) dependent single-stranded DNA cleavage |
| References | 1. David R Liu, et al. “Enhanced prime editing systems by manipulating cellular determinants of editing outcomes”. Cell. 2021 Oct 28;184(22):5635-5652.e29. 2. David R Liu, et al. “Phage-assisted evolution and protein engineering yield compact, efficient prime editors”. Cell. 2023 Aug 31;186(18):3983-4002.e26. |
| Purity | ≥90% by SDS-PAGE, 86% by SEC-HPLC |
| Residual DNase | Non-specific DNase activity |
| Endotoxin Level | Control <10EU/mg |
| Expression System | Recombinant Cas9-based fusion with three NLS expressed by E.coli |
| Species | S. pyogenes |
| Tag | C-His tag |
| Molecular Weight | 243.359KDa |
| Storage & Stability | PEmax is supplied with storage buffer (50 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.5, at 25°C) and recommended to be stored at -80°C. |
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