Standard curve of SARS-CoV-2 Nucleocapsid Protein LFA Assay.
The LFA assay is developed by using SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse(GenScript, A02135) and MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N34), mAb, Rabbit(GenScript, A02136) as the capture and detection antibodies, respectively.
The combination shows good dose response of fluorescence intensity and concentrations of SARS-CoV-2 Nucleocapsid protein in LFA assay.
Nucleocapsid protein concentration was nicely correlated with fluorescence intensity.
Western Blot of wild-type and delta variant Nucleocapsid protein cell lysates with MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N34), mAb, Rabbit (GenScript, A02136).
M: Protein Marker (GenScript, M00521)
Lane 1: 3 μg Wild-type N protein cell lysate
Lane 2: 3 μg Delta N protein cell lysate
Primary Antibody:
MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N34), mAb, Rabbit (GenScript, A02136) 1 μg/ml
Secondary Antibody:
Goat Anti-Rabbit IgG-HRP (GenScript, Cat No. A00131)
BLI (Biolayer interferometry) binding affinity measurements of MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N34), mAb, Rabbit (GenScript, A02136) to SARS-CoV-2 Nucleocapsid protein of wild type and delta variant.
MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N34) captured on Protein A sensors surface can bind wild-type or delta variant with dissociation constant (KD) of 2.25E-09M and 6.83E-10M.
BLI (Biolayer interferometry) binding affinity measurements of MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N34), mAb, Rabbit (GenScript, A02136) to nucleocapsid protein of SARS-CoV-2 omicron variant.
MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N34) captured on Protein A sensors surface can bind omicron variant with dissociation constant (KD) of 6.73E-10M.
MonoRab? SARS-CoV-2 Nucleocapsid Antibody (N34), mAb, Rabbit
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