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TRIM32 inhibits Venezuelan Equine Encephalitis Virus Infection by targeting a late step in viral entry

biorxiv. 2024-06; 
Yifan Xie, Jie Cao, Shuyi Gan, Lingdong Xu, Dongjie Zhang, Suhong Qian, Feng Xu, Qiang Ding, John W Schoggins, Wenchun Fan
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Proteins, Expression, Isolation and Analysis … analysis, the enriched proteins were separated on a gradient gel (GenScript, 4–20% 440 precast polyacrylamide gel) at 120V for 5~10 min, until the protein ladder ran ~1cm into gel. … Get A Quote

摘要

Alphaviruses are mosquito borne RNA viruses that are a reemerging public health threat. Alphaviruses have a broad host range, and can cause diverse disease outcomes like arthritis, and encephalitis. The host ubiquitin proteasome system (UPS) plays critical roles in regulating cellular processes to control the infections with various viruses, including alphaviruses. Previous studies suggest alphaviruses hijack UPS for virus infection, but the molecular mechanisms remain poorly characterized. In addition, whether certain E3 ubiquitin ligases or deubiquitinases act as alphavirus restriction factors remains poorly understood. Here, we employed a cDNA expression screen to identify E3 ubiquitin ligase TRIM32 as a nov... More

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