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An engineered (CAGA)-EGFP cell-based biosensor for high-content and accurate detection of active TGF-β

Biosens Bioelectron. 2022-11; 
Qian Ruan, Xuezhen Lin, Lixia Wang, Niu Wang, Yu Zhao, Hao Wang, Fu-Ying Tian, Ning Hu, Ying Li, Bo Zhao
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis … sorter (Biorad) after stimulation with active TGF-β1 (GenScript, Z03411). The enriched … assays. Therefore, we chose Expi293 F cell line as the basis for constructing our biosensor. … Get A Quote

摘要

Transforming growth factor-β (TGF-β) regulates multiple fundamental physiological processes and is closely related to severe diseases such as cancer, fibrosis, immune disorders and cardiovascular diseases. TGF-β is thus an important biomarker for clinical diagnosis and prognosis, and a crucial target for therapeutics development. Here we describe a high-content, serum-free, easy-to-use, and cost-effective (CAGA)-EGFP cell-based biosensor for accurate measurements of active TGF-β. Together with non-destructive and continuous measurement protocol and data processing method established here, the biosensor is capable of detecting active TGF-β1 in the range of 0.024-6.25?ng/mL concentration with >91% accuracy ... More

關(guān)鍵詞

(CAGA)(12), Cell-based biosensor, EGFP, High-content, High-throughput screening, Serum-free, TGF-β
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