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Effect of Induction Time on the Proliferation and Differentiation of Induced Schwann-Like Cells from Adipose-Derived Stem Cells

Cell Mol Neurobiol. 2020; 
Wong CW, Xu Y, Liu X, Xu S, Zhang Y, Zhu Z, He B.
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Recombinant Proteins … 10% FBS in DMEM/F12; and SC induction solution containing 10% FBS, 5 μM forskolin (SIGMA), 10 ng/ml basic fibroblast growth factor (bFGF; PeproTech, USA), 5 ng/ml platelet-derived growth factor (PDGF; ProSpec, USA), and 200 ng/ml heregulin (GenScript, China) in DMEM … Get A Quote

摘要

To compare how different?induction time takes effect?on the proliferation and secretion ability of adipose-derived stem cell (ADSC)-induced Schwann-like cells (iSCs), ADSCs were isolated from healthy adult female rats. Flow cytometry (FCM) was performed to detect the ADSC-positive markers CD29, CD44, and CD90 and the negative marker CD45. iSC induction medium was used to culture the ADSCs. S-100, GFAP, MBP, and P75 were detected by immunofluorescence staining to identify iSC differentiation. Cell morphological changes were observed by an inverted microscope after induction. An MTS assay was used to evaluate the cell proliferation ability. Western blot analyses of caspase-3/cleaved caspase-3 and FCM were appli... More

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