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Expression, Purification, and Properties of a Human Arachidonoyl-Specific Isoform of Diacylglycerol Kinase.

Biochemistry. 2017; 
Jennings William,Doshi Sejal,Hota Prasanta Kumar,Prodeus Aaron,Black Stephanie,Epand Richa
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Catalog Antibody ....) antibody (GenScript, Catalog No: A00186- 100). After washing in blocking buffer, membranes were.... further incubated with horseradish peroxidise-conjugated goat anti-mouse antibody (GenScript, Catalog Get A Quote

摘要

Diacylglycerol kinase ε (DGKε) catalyzes the phosphorylation of diacylglycerol, producing phosphatidic acid. DGKε demonstrates exquisite specificity for the acyl chains of diacylglycerol. This contributes to the enrichment of particular acyl chains within the lipids of the phosphatidylinositol cycle. Phosphatidylinositol is highly enriched with 1-stearoyl-2-arachidonoyl, which is important for maintaining cellular health. Dysregulation of DGKε perturbs lipid signaling and biosynthesis, which has been linked to epilepsy, Huntington's disease, and heart disease. Recessive loss-of-function mutations in the DGKε gene cause atypical hemolytic uremic syndrome. Because DGKε has never been purified, l... More

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