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Poly(I:C) source, molecular weight and endotoxin contamination affect dam and prenatal outcomes, implications for models of maternal immune activation.

Brain Behav Immun. 2019-08; 
KowashH M,PotterH G,EdyeM E,PrinssenE P,BandinelliS,NeillJ C,HagerR,Glazie
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Molecular Biology Reagents … 2.6. Endotoxin concentration. Endotoxin concentration in poly(I:C) samples was measured by ToxinSensor TM Chromogenic LAL Endotoxin Assay Kit (GenScript, USA; catalogue number L00350) according to the manufacturer's instructions. 2.7. Molecular weight analyses … Get A Quote

摘要

The viral mimetic polyinosinic:polycytidylic acid (poly(I:C)) is increasingly used to induce maternal immune activation (mIA) to model neurodevelopmental disorders (NDDs). Robust and reproducible phenotypes across studies are essential for the generation of models that will enhance our understanding of NDDs and enable the development of improved therapeutic strategies. However, differences in mIA-induced phenotypes using poly(I:C) have been widely observed, and this has prompted the reporting of useful and much needed methodological guidelines. Here, we perform a detailed investigation of molecular weight and endotoxin variations in poly(I:C) procured from two of the most commonly used suppliers, Sigma ... More

關鍵詞

Endotoxin,Fetal brain,Fetus,IL-6,Litter size,Maternal immune activation,Molecular weight,Placenta,Poly(I:C),Sex differe
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