The T7E1 assay to detect the cleavage efficiency of control HPRT gRNA with Cas9 protein.

GenCrispr T7 Endonuclease I

T7 Endonuclease I (T7E1) recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, hetero duplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5′ to the mismatch. The protein is the product of T7 gene 3. GenCrispr T7 Endonuclease I is a fusion protein produced from E.coli.

產(chǎn)品編號(hào)	Z03396
規(guī)格	250 U (10000 U/ml) 1250 U (10000 U/ml) 大單詢價(jià)
數(shù)量
價(jià)格	￥550


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Description

T7 Endonuclease I (T7E1) recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, hetero duplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5′ to the mismatch. The protein is the product of T7 gene 3. GenCrispr T7 Endonuclease I is a fusion protein produced from E.coli.

Synonyms

T7E1

Note

T7 Endonuclease I is a structure-selective enzyme. It acts on a variety of DNA substrates with different specific activities. It is important to control the amount of enzyme and the reaction time used for cleavage of a particular substrate. Temperatures above 42°C cause an increase in nonspecific nuclease activity and should be avoided.

應(yīng)用指導(dǎo)

Resolve four-way junction or branched DNA.
Detect or cleave hetero duplex and nicked DNA.
Randomly cleave linear DNA for shot-gun cloning.
Detect gene mutagenesis and SNPs, for cleavage efficiency assays induced by TALEN,CRISPR/CAS9 or other gene editing tools.

產(chǎn)品屬性

Storage & Stability

GenCrispr T7 Endonuclease I is supplied in 1X storage buffer (200 mM NaCl,20 mM Tris-HCl（pH 7.5）, 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton X-100 and 50% glycerol). The recommended storage temperature is -20°C.

圖例

The T7E1 assay to detect the cleavage efficiency of control HPRT gRNA with Cas9 protein.

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GenCrispr T7 Endonuclease I

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