| Specificity |
This product is
specific for Human CD28 |
| Host Species |
Mouse |
| Immunogen |
Recombinant Human
CD28 |
| Conjugate |
Unconjugated |
|
ex vivo cell culture process; Flow cytometry |
| Form |
Liquid |
| Storage Buffer |
Supplied in 10 mM
Sodium Citrate, 150 mM NaCl, pH 6.5. |
| Concentration |
5 mg/ml |
| Storage Instructions |
The product remains
stable at -70 ± 10 °C. Avoid repeated freeze-thaw cycles. Please refer to the
COA for specific expiry date. |
| Isotype |
Mouse IgG1κ |
| Clonality |
Monoclonal |
| Clone ID |
F105 |
Jurkat/IL-2-Luc Promoter cells were co-stimulated with a serial of concentrations of GMP Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. GMPA02213) and GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. GMPA02199) for 6 h. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway.
Accelerated stability test of GMP Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. GMPA02213) at 25 ℃ showed that this product is stable at 25 ℃ for 30 days and maintained high purity and potency.
Freeze-thaw cycle stability test of GMP Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. GMPA02213) showed that this product is stable for 5 times of freeze-thaw cycles and maintained high purity and potency.
| 外觀 |
Clear, colorless liquid |
| pH |
6.5 ± 0.5 |
| Concentration |
5.0 ± 0.5 mg/ml tested by A280 nm |
| Purity |
≥ 95% as analyzed by SEC-HPLC |
| Sequence |
The coverage of the light chain and heavy chain sequence was 100%
consistent with the theoretical amino acid sequence by mass spectrometry (MS) |
| Residual HCP |
≤ 100.0 ng/mg by ELISA method |
| Residual HCD |
≤ 10.0 pg/mg by quantitative PCR method |
| Residual Protein A |
≤ 10.0 ng/mg by ELISA method |
| Endotoxin Level |
< 0.1 EU/mg by gel clotting method |
| Sterility |
Sterile |
| Reactivity |
Qualified |
| Target Background |
CD28 (Cluster of
Differentiation 28) is one of the proteins expressed on T cells that provide
co-stimulatory signals required for T cell activation and survival. It plays an
important role in cell proliferation and cytokine production and promotion of
T-cell survival. It also enhances the production of IL-4 and IL-10 in T cells
in conjunction with TCR/CD3 ligation and CD40L co-stimulation. |
For laboratory
research and ex vivo cell isolation
and culture applications. Direct human use, including taking orally and
injection and clinical use are forbidden.
